Simple Novel Spectrophotometric and Spectrofluorimetric Methods for Determination of Some Anti- hypertensive Drugs

Accurate, precise and selective spectrophotometric and spectrofluorimetric methods were developed and subsequently validated for determination of Torasemide (I), Irbesartan (II) and Olmesartan medoxomil (III), where (I) could be determined in presence of its acidic-degradate as stability indicating method, utilizing derivative ratio spectrophotometry, also in human plasma it could be determined by spectrofluorimetric method, (II) could be determined in a binary mixture with Hydrochlorothiazide (HCTZ) by simultaneous determination, utilizing ratio subtraction and spectrofluorimetric techniques, while (III) could be determined in presence of its alkaline-degradate as stability indicating method, utilizing derivative ratio and pH-induced difference spectrophotometric technique, also in a binary mixture with Hydrochlorothiazide (HCTZ), it could be determined by simultaneous determination, using ratio subtraction and spectrofluorimetric methods. All the proposed novel methods were validated according to International Conference of Harmonization (ICH) guide lines and successfully applied to determine the mentioned studied drugs in pure form, in laboratory prepared mixtures and in pharmaceutical preparations. The obtained results were statistically compared to the reference methods of analysis [for I, II and III, respectively] and no significant difference were found. [M. Farouk, O. Abd EL-Aziz, A. Hemdan, M. Shehata. Simple Novel Spectrophotometric and Spectrofluorimetric Methods for Determination of Some Anti-hypertensive Drugs. Journal of American Science 2011;7(1):300-]. (ISSN: 1545-1003). http://www.jofamericanscience.org.